Vacuum Filtration for Filter Plate Assays | 101
This step requires the Y-connector waste tubing which is provided to connect the
control module to the washer’s main waste system.
1. Remove the main waste system tubing from the Vacuum port on the back of the
washer (waste tube with green bands on both ends), if it is installed. Plug it into
the barbed fitting of the Y-connector waste tubing.
2. Connect the branch of the Y-connector that does not have a green band (tube
with no tape) to the control module port labeled To Waste Line.
3. Connect the other end of the Y-connector (with green band), to the Vacuum port
on the back of the washer.
Connect the Vacuum Filtration Control Module to the Washer
Connect the supplied cable from the round port labeled To Filtration Valve
Control on the control module to the corresponding Vacuum Filtration Control port on
the back of the instrument.
Enable Vacuum Filtration
LHC 405 LSKeypad
1. Select Tools>Instrument
Utilities>Washer
2. Select installed for the Vacuum
Filtration Module.
1. Press Setup Menu.
2.
Select g>ADVANC>VACFIL.
3. Select Yes for Vacuum Filtration
Module.
Create a Vacuum Filtration Protocol
You may want to begin the protocol with a Shake step to suspend the beads.
LHC: Keypad:
1.
Click (or select File>New).
2. Select the plate type.
3. Specify a Protocol Name.
4.
Click
5. Click Show Wash Options
6. Fill the checkbox for Vacuum Filtration
1. At the main menu, select
DEFINE>CREATE and assign a
unique name to the protocol.
2. Select the plate type.
3. Select ADD>WASH
4. Select ASPIR>VAC
5. Specify the duration to apply
BioTek Instruments, Inc.
To Next Page
Loading...
