Decontaminate Exterior Surfaces | 139
70% isopropyl alcohol (as a bleach alternative)
Deionized or distilled water
Priming plate
Safety glasses
Surgical mask
Protective gloves
Lab coat
Biohazard trash bags
Clean cotton cloths
Step-by-Step Decontamination Instructions:
l Decontaminate Exterior Surfaces below
l Decontaminate Tubing and Manifold on next page
l Alternate Decontamination Procedure for Tubing and Manifold on page 141
Decontaminate Exterior Surfaces
Caution!Be sure to check the percentage NaClO of the bleach you are using; this
information is printed on the side of the bottle. Commercial bleach is typically 10%
NaClO; in this case, prepare a 1:20 dilution. Household bleach is typically 5% NaClO; in
this case, prepare a 1:10 dilution.
The bleach solution is caustic; wear gloves and eye protection when handling.
1. Turn off the instrument and disconnect the power cord. Empty the waste bottle.
2. Prepare an aqueous solution of 0.5% sodium hypochlorite (NaClO, or bleach).
As an alternative, 70% isopropyl alcohol (or 70% ethanol) may be used if the
effects of bleach are a concern.
n Isopropyl alcohol is not recommended for removing proteins (such
as bovine serum albumin).
3. Moisten a cloth with the bleach solution or alcohol. Do not soak the cloth.
l Wipe the keypad (do not soak). Wipe again with a clean cloth moistened with
deionized or distilled water. Dry immediately with a clean, dry cloth.
l Remove the mist shield if it is attached. Wipe the inside and outside surfaces of the
mist shield.
BioTek Instruments, Inc.
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