Biotek 405 LS - How to Use the Adjust Utility- LHC

60 | Chapter 3: Operation
o
Select the Plate Type and assign a unique Protocol Name Limit the name to 16
alpha-numeric characters if you want to run the instrument using the keypad
only, i.e. disconnected from the computer..
o
Click a button in the Add Step area.
o
Define the parameters for the step in the dialog that opens.
o
Continue adding steps, if desired.
o
Save the file and/or click Run to execute the protocol.
Double-click a step in the protocol to open it for editing.
• Highlight a step and press Delete to remove it.
• Click and drag a step to change its sequence order.
How to shake the plate
Add a Shake/Soak step to the protocol to shake the plate.
1. In the Protocol Steps box, highlight the step you want the shake to follow, and
click the Shake/Soak button.
2. Define an adequate duration and intensity to sufficiently mix the fluids in the
plate.
Leave the Soak option disabled when you only want to mix the plate contents, i.e.
perform a stand-alone shake step. Alternatively, enable the option and set a
duration to delay the process to allow the fluids to interact.
n Soak is not the same as "submerge" the tips. You must define a prime step or
use the AutoPrime feature to soak the tips in the priming trough.
n Soak is equivalent to incubating the plate at room temperature or delaying the
protocol.
n Shaking and soaking the plate is also an option when defining a Wash step.
How to use the Adjust Utility- LHC
This page describes how to use the LHC to run the Adjust Utility. See Run the
Adjust Utility (Using the Keypad) on page 109.
n Learn about the Adjust Utility.
l Keep in mind the positional limits of the devices during operation.
405™ Microplate Washer LS