140 | Chapter 4: Maintenance
l Wipe the plate carrier, top surface of the instrument’s base, supply bottles and
tubing, and all exposed surfaces of the instrument.
4. Wait 20 minutes. Moisten a cloth with DI or distilled water.
l Wipe the inside and outside surfaces of the mist shield.
l Wipe the plate carrier, top surface of the instrument’s base, supply bottles, tubing,
bottle covers and all exposed surfaces of the instrument that have been cleaned
with the bleach solution or alcohol.
5. Use a clean, dry cloth to dry all wet surfaces.
6. Reassemble the instrument as necessary.
7. Discard the used gloves and cloths using a biohazard trash bag and an
approved biohazard container.
Decontaminate Tubing and Manifold
Predefined protocols to flush and soak the supply tubing and manifolds with
disinfectant, then flush the system with rinse fluid are installed onboard the
instrument and on the host computer during installation of the LHC:
o
DECONTAMINATE for the washer
When storing or shipping the instrument, the LONG_SHUTDOWN procedure (on
page 143) primes and soaks the instrument, and ends by pushing air through the
system. The parameters can be edited for optimum cleaning. For example, consider
using ethanol instead of air to complete the decontamination process.
n Two supply bottles are required for this procedure: one for disinfectant, and
one for rinse. Except Buffer Switching models require four bottles altogether,
two of each solution.
1. Empty the waste bottle.
2. Prepare an aqueous solution of 0.5% sodium hypochlorite (NaClO, or bleach).
3. Fill one or two supply bottles with at least 300 mL of bleach solution
(disinfectant).
4. Fill another supply bottle or two with at least 500 mL of deionized water (rinse).
When using Buffer Switching:
l Buffer valve C & D: Disinfectant
l Buffer valve A & B: Rinse solution (they will be reversed in the next
round)
405™ Microplate Washer LS
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