Washer Liquid Tests | 157
Test Solutions
Solution #1: Buffer Solution
Pipette 1 mL Tween 20 into 1 liter (1000
mL) of deionized or distilled water and
mix well.
or Pipette 10 mL of BioTek Wetting Agent* into
1 liter of deionized or distilled water and
mix well.
* BioTek Solution #1 100X Concentrate Wetting Agent 125 mL (PN 7773002)
contains 10% Tween 20 in deionized water and 0.01% Sodium Azide as a
preservative.
SOLUTION #2: Residual Test Solution
Mix 100 mL of Solution #1 with 0.050
grams of FD&C #1 blue dye.
or Mix 90 mL of Solution #1 with 10 mL
of BioTek Blue Test Dye*.
* BioTek Solution #2 10X Concentrate Blue Test Dye 125 mL (PN 7773001)
contains 5 grams per liter FD&C Blue #1, 0.1% Tween 20 in deionized water and
0.01% Sodium Azide as a preservative.
SOLUTION #3: Dispense Precision Solution for 96-Tube manifolds
Mix 1180 mL of deionized or distilled water with 20 mL of Solution #2.
SOLUTION #4: Dispense Precision Solution for 192-Tube manifolds
Mix 1420 mL of Solution #1 with 20 mL of Solution #2.
Dispense Precision Test (96)
n This test is designed for 96-Tube manifolds. Find the Dispense Precision Test
(192) on page 161.
n Find a list of the supplies you will need at Materials on page 155.
n Save the plate! When this test is complete, use the filled plate to perform the
Evacuation Efficiency Test.
n Keypad Users: The protocol names onboard the washer do not include the
W- prefix and some protocols include the model name, e.g. 405HT or
405Select, when appropriate.
1. Fill a supply bottle with 2 liters of deionized or distilled water; Buffer Switching
units use buffer valve A. Run the W-DAY_RINSE protocol two or three times to
prime the fluid lines and manifold.
BioTek Instruments, Inc.
To Next Page
Loading...
