30 | Chapter 2: Installation
Verify the Washer
1. Fill the washer’s supply bottle (bottle “A” for buffer switching models) with
approximately one liter of deionized water.
LHC Keypad
1. Select File > Open.
2. Open the 405 LS and the Prime and
Maintenance Protocols folders.
Open the applicable "Buffer" folder,
and then open W-DAY_RINSE.LHC
You may need to reset the Com
Port.
3. When ready, click the Run button
to prime the tubing and manifold
with deionized water.
4. When finished, close the program.
1. Select RUN at the main menu.
2. Scroll (press Options) to DAY_
RINSE. Press ENTER to confirm
selection of the protocol and press
START to run the protocol.
3. When the protocol is completed,
press Main Menu.
Run the Verify Test ("Q" Models Only)
If your 405 LS is equipped with BioTek's Verify™ Technology, run the test:
Important! Before running the test:
Make sure the 405 LS is primed and ready to run:
l Fully prime the tubing/system, e.g. run Day Rinse.
l If necessary, empty waste vessel and tighten waste bottle cap.
Prepare to run the Verify test:
l Fill the supply vessel with at least 100 mL dH2O or DIwater or buffer solution
1
.
l Put the Verify Test Plate on the standard-mag bead plate carrier (without a magnet)
2
in
the proper orientation: "Front" label is readable (well A1 in the back, left of the carrier).
See also: Handling the Verify Test Plate on page 73.
1
Do not use highly viscous fluids or wash buffers that are prone to leave significant residue on the plate.
2
Vacuum filtration assays: You must uninstall the vacuum filtration plate carrier and install the standard-
mag bead plate carrier to run the test. For this temporary purpose, you do not need to change the
instrument's plate carrier setting to run the Verify test.
405™ Microplate Washer LS
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