Target Instrument Settings | 69
Rate mm/sec
4 9.4 +1
4 CW 9.4
5 9.4 +2 Fastest
6 CW 14.7 Recommended rate
for Cell Wash
protocols
CW rates are specially designed travel rates that minimize turbulence in the wells
for Cell Wash protocols.
Standard rates (1-5) show two speeds as they approach the well because they slow
down to 1 or 2 mm/sec before reaching the aspirate height to provide more time to
aspirate the fluid, improving evacuation. Conversely, the CW rates do not change
speeds, they move into and out of the well as quickly as possible to limit
turbulence.
How to define a Final Aspiration step
By default, wash steps include a final aspiration step because a common objective
is minimizing the residual left in the wells during a wash cycle. You can enable,
disable, or modify the parameters of the final aspiration step.
n Important info for Vacuum Filtration Assays: Be sure to reset or disable the
Final Aspiration step to match your assay requirements.
Edit the Wash step to modify the Final Aspiration:
LHC Users: Keypad Users:
1. Double click the step in the
protocol to open it for editing.
2. Fill the checkbox to enable Final
Aspirate and click the Definition
link to define the parameters to suit
your assay.
1. Edit the Wash step or stand-alone
Aspirate step: Define>Edit>select
protocol, and if necessary press
Options to scroll to the desired
step.
2. Select OPTS>POST and select Yes,
or modify the Final Aspirate step
by pressing Enter.
3. Define or modify the parameters
to suit your assay.
BioTek Instruments, Inc.
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