72 | Chapter 3: Operation
Verify Performance
n LHC Required for "Q" models:you must use BioTek's Liquid Handling
Control (LHC) software to run the Verify™ Technology clog detection feature.
Tools>Instrument Utilities>Verify Manifold>Run a New Test
BioTek's Verify™ Technology quickly detects clogged aspirate or dispense tubes.
Important! Before running the test:
Make sure the 405 LS is primed and ready to run:
l Fully prime the tubing/system, e.g. run Day Rinse.
l If necessary, empty waste vessel and tighten waste bottle cap.
Prepare to run the Verify test:
l Fill the supply vessel with at least 100 mL dH2O or DIwater or buffer solution
1
.
l Put the Verify Test Plate on the standard-mag bead plate carrier (without a magnet)
2
in
the proper orientation: "Front" label is readable (well A1 in the back, left of the carrier).
See also: Handling the Verify Test Plate on the facing page.
Run the Verify test:
LHC
1. Select Tools>Instrument Utilities>Verify Manifold and click Run a new Test.
l Buffer: Select the bottle to use, if applicable.
l Prime: optionally, prime the manifold tubes to correct for evaporation loss.
40 mL of fluid is dispensed.
l %CV Threshold: optionally, change the %CV to match your lab's standard for
QC tests: 5-15%. The default value of 5% is recommended.
2. Press Start to run the test.
3. Assess the results: Verify Results.
Do not discard the Verify Test Plate. Reuse it for as long as possible. See
Replacement Procedure for Verify Test Plate on page 134 if it is damaged.
1
Do not use highly viscous fluids or wash buffers that are prone to leave significant residue on the plate.
2
Vacuum filtration assays: You must uninstall the vacuum filtration plate carrier and install the standard-
mag bead plate carrier to run the test. For this temporary purpose, you do not need to change the
instrument's plate carrier setting to run the Verify test.
405™ Microplate Washer LS
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