Target Instrument Settings | 71
create cleaning turbulence (at the specified height). The manifold descends to
aspirate again and ends with a final dispense to fill the wells.
Buffer: Select the buffer to use, if applicable.
Bottom Wash Volume: Enter the volume of wash solution to dispense per well
during the bottom wash.
Flow Rate: Set the rate. Valid range is 3-11. The cell wash rates, 1 CW and 2 CW,
which use low-flow tubing, when available, are not recommended. Cell wash
options are designed for gentle washing, while bottom wash is designed for
vigorous washing.
Advanced Options
Parameters Description
Bottom Wash
Height:
Z-axis Position
The distance between the bottom of the aspirate
tubes and the carrier surface. The valid range is 12
to 175. The value in mm is displayed on screen.
Horizontal X-axis
Position
The left and right position of the dispense tubes
when the carrier is beneath the manifold.
Horizontal Y-axis
Position
The front-to-back carrier position (or Y axis) to align
the microplate with the manifold tubes during a
dispense. The range depends on the model.
Delay start of
Vacuum
until dispensing this volume. 3000 µL max
BioTek Instruments, Inc.