Biotek 405 LS - Evacuation Diagnostic Test

Washer Liquid Tests | 159
1. Fill a supply bottle with 2 liters of deionized or distilled water. Run the
Maintenance program W-DAY_RINSE two or three times to prime the tubing
and manifold.
2. Place a new 96-well microplate on the balance and zero the balance.
3.
Pipette or dispense 150 µL of Solution #1 into each well of the microplate.
4. Place the plate on the carrier and run the QC-96_EVAC_TEST protocol.This
protocol evacuates the wells, leaving a small amount of residual fluid.
5. When the program is completed, remove the plate and weigh it immediately
because evaporation will affect the results.This is the Total Residual Weight in
grams.
6. Visually inspect the plate and note if any wells appear to have considerably
more liquid than others.
7. Use the Evacuation Efficiency Test Worksheet to perform data reduction:
l Divide the Total Residual Weight by 96 to find the Mean Residual Weight.
l The Mean Residual Weight should be <= 0.002 g.
If the Mean Residual Weight is greater than 0.002 g, or if one or more wells
appear to have much more liquid than the others, the washer failed the test.
Troubleshoot as follows:
If the test fails once:
o
If the problem appears to be related to particular wells, clean those aspiration
tubes: run AutoClean and/or remove the manifold and thoroughly clean the tubes
with the stylus (See Remove and clean the washer manifold on page 131).
When finished, retry the test.
o
Failure of this test is commonly caused by improper aspiration tube placement
within the wells, usually because a microplate other than the recommended
Corning Costar
®
96 was used. If you must use a plate other than the Corning
Costar 96, modify the Aspirate Height, i.e., Z position, or horizontal X or Y position
parameters in a copy of the QC-96_EVAC_TEST protocol to correct this error.
After making this change, retry the test using a new microplate.
If the test fails a second time: Perform the Evacuation Diagnostic Test.
Evacuation Diagnostic Test
n Conduct this test if the standard Evacuation Efficiency Test fails twice. This
test will confirm which aspirate tube(s) may be clogged, or if the plate’s
alignment or position is the problem.
BioTek Instruments, Inc.