158 | Chapter 5: Qualification
2. Fill a supply bottle with 1200 mL of Solution #3; Buffer Switching units use
buffer valve A.
3. Run W-DAY_RINSE again to prime the washer with the solution.
4. Place a new 96-well microplate on the balance and zero the balance.
5. Place the plate on the carrier and run the QC-96_DISP_TEST. This protocol
dispenses 300 µL of solution to each well of the plate. It does not evacuate the
solution.
6. When the protocol is completed, carefully remove the plate. Place the plate on
the balance and record the Total Dispense Weight.
7. Read the plate in an optical reader (blank on air), using the dual-wavelength
method (630 nm - 450 nm), then print or export the results.
8. Use the Dispense Precision Test Worksheet to perform data reduction:
Tip: If you have a spreadsheet software program, enter/export all 96 values into a
spreadsheet and apply your program’s Standard Deviation function (e.g., Microsoft®
Excel’s STDEV).
a. Calculate the Standard Deviation.
b. Calculate the sum of the OD values for all 96 wells, then divide by 96 to
determine the Mean OD for the plate.
c. Calculate the %CV: (Standard Deviation / Mean OD) * 100.
The %CV should be <= 3.0.
If the %CV is greater than 3.0, one or more dispense tubes may need to be cleaned.
Run AutoClean, if available, and/or remove the manifold and use the stylus to
clean the dispense tube(s) giving lower-than-average absorbance readings. When
finished, re-prime the washer and retry the test.
9. When finished, prime with deionized water to flush out the dye solution.
Evacuation Efficiency Test (96)
n This test is designed for 96-Tube manifolds. Find the Evacuation Efficiency
Test (192) on page 162
n If you tared the balance at the start of the Dispense Precision Test, use the
plate from that test here; skip steps 1-3.
Keypad Users: The protocol names onboard the washer do not include the W- prefix and
some protocols include the model name, e.g. 405HT or 405Select, when appropriate.
405™ Microplate Washer LS
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