Vacuum Filtration Evacuation Efficiency Test | 165
Vacuum Filtration Evacuation Efficiency Test
This test is designed for the 405 LS with the Vacuum Filtration accessory. Install the vacuum
filtration plate carrier to perform the test. Make sure the instrument setting for Plate Carrier is
set correctly. Use the plate size most commonly used in your lab.
n Perform this test during the initial and annual OQ and the monthly PQ.
1. Fill a supply bottle with 2 L (two liters) of deionized water. Run W-DAY_
RINSE to prime the fluid lines and manifold.
2. Place a new microplate on the balance and zero the balance:
o
96-well 0.45 µm filter plate: Millipore MSHVN4550 96 is recommended.
o
384-well 1.2µm filter plate: Millipore MZFCN0W10 is recommended.
3. Run the predefined protocol to first dispense deionized or distilled water into
each well of the microplate and then evacuates the wells:
Plate Predefined Protocol Volume Vacuum Time
96-well QC-96_VAC30_TEST 300 µL/well 30 seconds
384-well QC-384_VAC10_TEST 80 μL/well 10 seconds
4. When the protocol is finished, remove the plate, and blot the bottom of the
plate on a paper towel to remove any droplets.
5. Weigh the plate immediately. This is the Total Residual Weight, in grams.
6. Visually inspect the plate and note if any wells appear to have considerably
more liquid in them than others.
7. Use the “Vacuum Filtration Evacuation Efficiency Test” worksheet at the end
of this chapter to record the results:
Residual Weight should be:
o
<= 1.2 grams for a 96-well plate;
o
<= 4.0 grams for a 384-well plate.
n While BioTek recommends using the Millipore filter-bottom plate listed above
in this test, you may substitute other manufacturers’ filter-bottom plates that
you are familiar with. However, using a substitute plate may vary your test
results.
If the Residual Weight is greater than specified, the washer failed the test. Make
sure a tight seal was maintained between the filter plate and plate carrier:
BioTek Instruments, Inc.
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