Introducing the 405 LS Keypad | 51
3. Press the Options key to toggle between filling the column or not. When the
image of the column is filled it will be dispensed to. Conversely, when the
column image is blank or unfilled, the column will not be dispensed to.
4. Press Enter to save the settings and continue.
Wash Step Format: Plate or Sector
When washing a high-density plate you can choose between two processing
methods, Plate or Sector:
l Plate format performs each wash cycle to the entire plate before it starts the next
cycle.
l Sector format performs the entire wash step to each sector of the plate before it
moves to the next sector. A sector is defined by the manifold's footprint:
o
96-tube manifolds process 384-well plates in 4 evenly spaced sectors (quadrants);
o
192-tube manifolds process 384-well plates in two sectors: even and odd
numbered columns.
Use sector mode when you are concerned about the fluid drying out before the
procedure is completed. To learn more See About Wash Processing Patterns on page
111
How to shake the plate
These instructions are for keypad control.
The shake command is tied to the Soak option. These instructions apply to soaking
or incubating the plate at room temperature, as well as shaking.
There are two ways to specify a shake period:
l During a wash cycle: To shake (and/or soak) in between every wash cycle,
select OPTS when defining Wash Step Parameters and then select MIDCYC,
and follow the prompts to specify parameters.
l Shake step: create a protocol to shake the plate, it can be a one-step, shake-
only protocol, or you can ADD a Shake step before or after another step.
n See Create or Edit a Protocol (Keypad Only) on page 48
n Soak is not the same as "submerge" the tips. You must define a prime step or
use the AutoPrime feature to soak the tips in the priming trough.
n Soak is equivalent to incubating the plate at room temperature or delaying the
protocol.
BioTek Instruments, Inc.
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