160 | Chapter 5: Qualification
1. If you have not already done so, repeat steps 2 through 7 of the standard
Evacuation Efficiency test, using Solution #2 for the dispense fluid. Be sure to
recalculate the Mean Residual Weight.
2.
Pipette up to 300 µL of Solution #1 into each well, on top of the residual
solution.
3. Shake the plate to achieve uniform distribution of the remaining dye in each
well.
4. Read the plate in an optical reader (blank on air), using the dual-wavelength
method (630 nm - 450 nm), then print or export the results.
5. Use the Evacuation Efficiency Test Worksheet to perform data reduction:
l Calculate the sum of the OD values for all wells, then divide by 96 to
determine the Mean OD for the plate.
l Divide the Mean OD by the Mean Residual Weight (from step 1), to find the
Residual Factor.
l For each well, divide its OD value by the Residual Factor to find its Residual
Weight.
Each well’s Residual Weight should be <= 0.002 g.
If one or more wells have a Residual Weight greater than 0.002 g, review the data to
determine which well, or wells, is causing the problem.
o
If the problem appears to be related to particular wells, clean the associated
aspiration tubes: run AutoClean and/or remove the manifold and thoroughly
clean the tubes with the stylus. (See Remove and clean the washer manifold
on page 131.) When finished, retry the test.
o
If the problem appears to be related to a particular region, edge, or corner of the
plate, review the alignment and flatness of the plate on the carrier.
Please do not adjust the carrier adjustment screws! Contact BioTek if you suspect an
alignment problem, contact BioTek TAC.
o
For additional suggestions, See Troubleshooting on page 180 .
o
If the test continues to fail, contact BioTek Instruments.
Buffer Switching Dispense Test
This procedure tests all the buffer valves.
n The Dispense Precision test must pass before the test for Valves A-D can be
performed.
405™ Microplate Washer LS
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