76 | Chapter 3: Operation
Corrective Action
recheck the instrument setup:
l System fully primed;
l Sufficient/correct fluid supply;
l Vacuum pump working;
l Washer on a level surface;
l Supply vessels on same or higher elevation than
washer;
l Supply vessel filter (in bottle) unclogged;
l Waste bottle cap tightened;
l Inline vacuum filter unclogged.
See Remove and clean
the washer manifold
on page 131
Note All or many tubes: if the plate map shows:
l all the aspirate tubes as clogged: check for a
vacuum leak
l clusters of dispense tubes as clogged: check fluid
supply/fully primed system
About Verify™ Technology
BioTek's Verify™ Technology uses an ultrasonic liquid-level sensor to quickly test
aspirate and dispense performance. In less than 5 minutes, the Verify routine will
give you supreme confidence that assay plates will be washed according to your
specifications or it will identify any clogged manifold tubes that need cleaning.
Our Verify Technology can significantly decrease the need for routine instrument
quality control using fluorescence- or absorbance-based dyes and a microplate
reader, as the test can be performed with dH2O/DI water and most wash buffers
by the washer itself. You can run the Verify test prior to each assay, at the
beginning or end of every day, or at the frequency that best fits your lab's current
practices. However, use of the Verify Technology does not alter the recommended
instrument qualification schedule, i.e. dispense precision and evacuation efficiency
testing should be performed monthly to confirm the washer is performing to
specifications. See Sensitivity: Verify Sensor vs. Absorbance Reader on page 78.
See Verify™ Technology Flowchart Diagram on previous page for a graphical depiction
of the process.
The Verify routine runs a pre-check and five tests:
405™ Microplate Washer LS
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