58 | Chapter 3: Operation
Protocols are considered valid when an instrument can successfully perform the
protocol. The LHC will run a protocol even when the instrument settings do not
match the physical configuration of the instrument. For example, a protocol with
instrument settings that include Buffer Switching can be run by an instrument
without Buffer Switching when none of the steps actually call for different buffer
valves, i.e. all steps use the same buffer.
Validate versus Run
Validate checks the action steps against the protocol's Target
Instrument Settings.
Run talks to the instrument to check the action steps against the
instrument's onboard settings.
Validate will catch errors when the Instrument Settings have been changed after the
protocol steps have been defined and there is a mismatch. Run performs a similar
validation before executing the protocol. Errors are not reported unless the steps
cannot be performed.
Target Instrument Settings
n For LHCusers only.
Click the Settings link in the main workspace
Actual Instrument or Simulated Instrument? That is the question for the Target
Instrument Settings dialog.
When the 405 LS is:
l connected to the computer: it is best to "Get (the) actual settings" from it;
l not connected to the computer: you must define the settings.
One vs. Multiple Instruments
l If you are running only one instrument, always "Get the actual settings" to identify the
exact configuration of your 405 LS to ensure it can successfully perform the wash
protocols.
l If you are managing multiple BioTek instruments (or one instrument with multiple
configurations): you can create and save a "settings file" for each instrument to help
create protocols for that instrument when you are not connected to it. See below.
The "instrument settings" tell the LHC what the instrument can do, e.g. fill a 384-
well plate or not. It is impossible to create a protocol without this information. Read
405™ Microplate Washer LS
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