56 | Chapter 3: Operation
LHC Protocols
BioTek provides predefined protocols for maintenance routines, instrument
verification, and general samples for common applications like serial dilutions.
Review the Predefined Protocols on page 43
Customize the Protocols
Typically, you must modify the predefined protocols to match your instrument
configuration and to meet your assay requirements.
Instrument Settings: In addition to action steps, every protocol file contains
instrument settings, including COM port, manifold type, and so on. Edit the
protocol to match your instrument's COM Port and other configuration details:
l Customize the Predefined Protocols on page 41
l Power Users: If you create protocols for multiple instruments or for other LHC users,
read this more detailed description of how the 405 LS validates a protocol to be run on a
specific instrument.
n Recommended: Before changing a predefined protocol, select File>Save As
and give it a unique name. This practice preserves the custom protocol in the
case of a future upgrade.
File Location
The LHC installs the protocols in the Windows Common Applications Data Folder:
l Windows® XP: C:\Documents and Settings\All Users\Application Data\
l Windows® Vista™ and Windows 7: C:\ProgramData\
The file location path continues:
[CommonAppDataFolder]\BioTek\Liquid Handling Control v.#\Protocols\405 LS
Three folders are provided:
l \Prime and Maintenance: the recommended daily and periodic maintenance routines;
the 405 provides distinct maintenance protocols for models with Buffer Switching
(4Buffer) and those without (1Buffer);
l \QC: some of the quality control or performance verification procedures;
l \Samples: examples of common applications, including washing 96- and 384-well
plates, performing serial dilutions, and a cell wash protocol.
405™ Microplate Washer LS
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