Biomagnetic Separation - Magnetic Bead Assays | 89
Biomagnetic Separation - Magnetic Bead Assays
The microplate carrier supports placement of a magnet under the microplate. The
magnet induces magnetic beads to settle at the bottom of the wells to help retain
them during a wash protocol’s aspirate cycle.
The 405 LS supports standard microplates and these magnets available for
purchase from BioTek:
Plate Type Magnet PN
96-well 96 Flat Magnet 7103016
96 Ring Magnet 7102216
384-well 384 Flat Magnet 7103017
384 Ring Magnet 7102215
You can use a different magnet if it fits in the carrier and accommodates your
plates. Contact BioTek TAC or visit the Customer Resource Center at
www.biotek.com to obtain a drawing of the carrier with its dimensions.
Persons with pacemakers/implants should avoid direct contact. Keep all
magnetic media, watches, and sensitive electronic devices away from the magnets.
Credit cards, tapes, and disks can be erased in the presence of a magnetic field. Bodily
harm [pinching of hands and skin] can result if magnets are not handled correctly.
Maintain distance between two or more magnets.
Take advantage of the sample magnetic-bead protocols shipped onboard the 405 LS.
Copy W-Luminex_Mag_Flat_96, for example, and modify the parameters to suit your assay
requirements.
Handling and Cleaning the Magnets
For best magnet strength and bead retention, the bottom of the microplate must be
as close to the magnet as possible. We recommend using flat-bottom plates with
minimal support “webbing” between the sides of the outer wells and the plate
skirts.
Handle the magnets with care. Avoid direct contact with the magnet material. Keep
loose ferrous material away and do not attempt to disassemble.
The magnet should be stored in a cool, dry environment and should be cleaned
with a damp cloth and mild detergent when exposed to harsh solvents. Do not
autoclave.
To install the magnet in the proper orientation:
BioTek Instruments, Inc.
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