AutoClean the Washer | 125
2. Run the DAY_RINSE protocol three times.
3. Perform your regular Overnight/Multi-Day Maintenance routine.
Weekly or As Needed use NaOH and HCl to remove proteins:
1. Flush the system with 0.1-0.5 N* NaOH (sodium hydroxide), followed by
neutralization with an equivalent normality (0.1-0.5 N) of HCl (hydrochloride).
2. Rinse well with deionized water to remove the HCl.
3. Run the applicable DAY_RINSE protocol three times with deionized water if
you plan to use the device immediately.
n * N = Normal solution, which contains 1 ‘gram equivalent weight’ (gEW) of
solute per liter of solution. The gram equivalent weight is equal to the
molecular weight expressed as grams divided by the ‘valency’ of the solute.
Alternatively use an Enzyme-Active Detergent:
1. Mix an enzyme-active detergent according to the manufacturer’s directions to
fill a four-liter supply bottle. Connect the bottle to the washer’s Buffer valve A.
Connect a bottle of deionized or distilled water to Buffer valve B to rinse the
tubing.
2. Run the DECONTAMINATE protocol, as appropriate.
3. LHC users: Respond to the Delay message, “Connect a bottle of water…”, leave
the detergent bottle connected and when ready, press Continue.
4. When the protocol is completed, connect a bottle containing four liters of
deionized water and run DAY_RINSE three times to flush the system.
AutoClean the Washer
Learn About AutoClean on next page
Some models of the 405 LS feature BioTek's Ultrasonic Advantage™ for easy and
thorough cleaning of the wash manifold. Instruments with AutoClean capability are
easily identified by the stainless steel priming reservoir built into the instrument's
base, under the wash manifold.
Warning! Ultrasonic energy is present in the cleaning reservoir when the AutoClean
program is running. Do not put your fingers in the bath! Ultrasonic energy can harm
human tissue.
BioTek Instruments, Inc.
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