Biotek 405 LS - AutoClean the Washer

Washer Settings | 105
Testing at BioTek confirmed expectations: small pore filter plates and highly
viscous fluids require increased vacuum and/or longer aspiration durations to
evacuate the wells.
Here are the results of our tests using 25% horse serum to give you a starting point
for your experimentation.
Plate and Dispense Volume Vent Plug
Aspiration
Time
Millipore 96-well 0.45µM filter plate;200 µL/well sample
volume
Beige
w/hole
12.9 sec
Gray 8.8 sec
Black 7.1 sec
Beige 6.4 sec
Millipore 96-well 1.2 µM filter plate; 200 µL/well sample
volume
Beige
w/hole
6.2 sec
Gray 4.7 sec
Black 4.6 sec
Beige 3.9 sec
Millipore 384-well 1.2 µM filter plate; 100 µL/well sample
volume
Beige
w/hole
8.9 sec
Gray 6.5 sec
Black 6.0 sec
Beige 4.7 sec
Washer Settings
Touch screen: Instrument>Options
LHC users: Tools>Instrument Utilities>Washer
Manifold Selection
After physically changing the manifold, tell the 405 LS which manifold is installed:
l 96-tube: single manifold for 96-well plates only or dual manifolds to process 96- and
384-well plates.
l 192-tube: only 384-well plates.
Magnet Height Offset
BioTek Instruments, Inc.