Define Instrument Settings | 27
Standard Vacuum Pump Users
n Do not perform this step when using the High Flow vacuum pump (PN
7100754). High flow pumps are recommended when aspirating 384-well plates
with non-surfactant wash buffers (e.g., pure deionized or distilled water).
Perform this step ONLY if you are using the “standard” vacuum pump (PN
7103024): increase the Vacuum Dissipate Delay to match your waste container: 1
second per liter. For example, if you have a 10 L waste bottle, set the delay to
10 seconds.
Using the LHC Using the Keypad
1. Select Tools>Instrument Utilities.
2. Under General Settings, increase the
Vacuum Dissipate Delay to match
your waste container: 1 second per
liter.
3. Click Send to download this new
setting to the instrument.
4. Click Exit to return to the main
screen.
1. Press Setup Menu.
2.
g Select the arrow (for more
options) then, ADVANC.
3. Select VACDIS.
4. Use the number pad to set the
Vacuum Dissipate Delay to
match your waste container: 1
second per liter.
5. Press Main Menu upon
completion.
Define Startup Preferences (LHC users only)
You can save enormous time creating protocols by following these steps to define a
New Protocol template and use it at startup.
Create a protocol template
1.
Click the New button or select File>New.
2. Click Name. Select the 405 LS and define its Port and Settings.
3. Optionally, select the Plate Type, fill in the text fields, and add any steps that
you want all new protocols to include.
4.
Click Save and assign a unique name, e.g. Template.LHC.
5. Select Tools>Preferences>New Protocol.
6. Select the button for Protocol selected below to use as a template.
7. Click selected and select the protocol you created as a template.
BioTek Instruments, Inc.
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