70 | Chapter 3: Operation
Add a secondary aspiration to a wash cycle
When too much residual is left in the wells during a wash cycle, you can add a
secondary aspiration to reduce it. Secondary aspiration can be performed
immediately after each aspiration in a wash cycle and/or after the final aspiration
step.
n Secondary aspiration is also called Crosswise aspiration because it is typically
performed in a different location within the well. Reposition the tubes by
defining different X-, Y-, and/or Z-axis positions when enabling the secondary
aspiration for the most effective evacuation of the wells.
Edit the Wash step or stand-alone Aspirate step to add a secondary aspiration:
LHC Keypad
1. Double click the step in the
protocol to open it for editing.
2. Click Advanced Options (for
Aspirate) and enable Perform
Secondary Aspirate.
3. Apply a second aspiration to the
Final Aspirate, also, for best
evacuation results.
4. Optionally, adjust the position of
the manifold as it addresses the
wells.
1. Edit the Wash step or stand-alone
Aspirate step: Define>Edit>select
protocol, and if necessary press
Options to scroll to the desired
step.
2. Select ASPIR and press Enter until
Secondary Aspirate is offered.
And/or select OPTS>POST to add a
second aspiration to the Final
Aspirate.
3. Select YES to perform a second or
crosswise aspiration.
4. Specify a crosswise position for the
manifold as it addresses the wells.
For example, you may want to adjust the X- or Y- axes to get to hard-to-reach areas
of certain types of wells. Or, you may want to lower the height (reduce the Z-axis),
to better evacuate fluid.
Run the Adjust Utility: To determine the precise positioning of the dispense and
aspirate tubes.
Bottom Wash
Bottom washing adds an initial dispense/aspirate sequence, another wash cycle, to
the specified number of cycles. Fluid is simultaneously dispensed and aspirated to
405™ Microplate Washer LS
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