Decontaminate Tubing and Manifold | 141
Without Buffer Switching, connect the bottle containing disinfectant to the
Fluid In port.
5. Reconnect the power cord and turn on the instrument.
6. Run the decontamination protocols.
Preparing to run DECON or W-DECONTAMINATE:
With Buffer
Switching
Connect the supply bottles this way:
• Buffer bottles C and D: Disinfectant
• Buffer bottles A and B: Rinse solution
After disinfecting channels C and D, you will reverse this assignment
to disinfect channels A and B.
Keypad:Run DECON_STEP1, DECON_STEP2, and DECON_
STEP3.
LHC: At the prompt/delay, change bottles.
Without Buffer
Switching
Connect the disinfectant bottle to the Fluid In port.
Keypad:Run DECON_STEP1 followed by DECON_STEP2.
LHC: At the prompt/delay, change bottles.
Alternate Decontamination Procedure for Tubing and Manifold
If you are unable to run the decontamination protocols due to a system failure,
perform the following alternate decontamination procedure to disinfect the internal
tubing and manifolds.
Caution!Be sure to check the percentage NaClO of the bleach you are using; this
information is printed on the side of the bottle. Commercial bleach is typically 10%
NaClO; in this case, prepare a 1:20 dilution. Household bleach is typically 5% NaClO; in
this case, prepare a 1:10 dilution.
1. Turn off the instrument and disconnect the power cord.
2. Remove the mist shield.
3. Remove the wash manifold(s) and end plate(s).
n For dual manifold models, hold the two manifolds (and end plates) together as
a single unit when removing and replacing them.
4. Prepare an aqueous solution of 0.5% sodium hypochlorite (bleach). As an
alternative, 70% isopropyl alcohol (or ethanol) may be used if the effects of
bleach are a concern.
n Isopropyl alcohol is not recommended for removing proteins (such as
bovine serum albumin).
BioTek Instruments, Inc.
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