Vacuum Filtration for Filter Plate Assays | 97
Vacuum Filtration for Filter Plate Assays
With the optional vacuum filtration accessory kit, you can process most standard-
size filter-bottom microplates.
To perform filter plate assays:
l Install the side bracket on next page
n Follow instructions provided with the accessory kit.
l Set up the Vacuum Filtration module
l Install Vacuum Filtration Plate Carrier on page 103
l Create a Vacuum Filtration Protocol on page 101
Maintenance guidelines: Maintaining the Vacuum Filtration System on page 133
n For the best results, take a few moments to learn how to Control the Vacuum
Level on page 104.
Take advantage of the sample vacuum filtration protocols shipped onboard the 405 LS.
Copy W-Luminex_Vac_96, for example, and modify the parameters to suit your assay
requirements.
Recommendations for best performance:
Here are some guidelines to achieve the best performance of your filter plate
assays:
l Do not use dry filter plates. If dry plates are required by the assay kit
instructions, turn off the Vacuum Filtration sensor to avoid process
interruptions.
l Shake the plate to suspend the beads before aspiration. Enable the wash
cycle option to shake the plate after the dispense and before aspiration. Also
consider creating a multi-step protocol that begins by shaking the plate.
l Experiment with the two parameters, aspiration time and vacuum level, to
determine the best combination of settings for your assay. Start with a brief
time period and low vacuum to avoid lodging the beads in the filter
material.
l Maintain consistent vacuum during the process with a tight seal:
o
Use new or defect-free filter plates and make sure they are seated perfectly in the
carrier;
o
Make sure all tubing is connected correctly, and leak-free.
BioTek Instruments, Inc.
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