162 | Chapter 5: Qualification
fluid lines and manifold.
2. Fill a supply bottle with 1440 mL of Solution #4; Buffer Switching units use
buffer valve A.
3. Run W-DAY_RINSE again to prime the washer with the solution.
4. Place a new 384-well microplate on the balance and zero the balance.
5. Place the plate on the carrier and run the QC-192_DISP_TEST protocol.This
protocol dispenses 80 µL of solution to each well of the plate.It does not
evacuate the solution.
6. When the program is finished, carefully remove the plate. Place the place on the
balance and record the Total Dispense Weight.
7. Read the plate in an optical reader (blank on air), using the dual-wavelength
method (630 nm - 450 nm), then print or export the results.
8. Use the Dispense Precision Test Worksheet, perform data reduction:
Tip: If you have a spreadsheet software program, enter/export all 384 values into a
spreadsheet and apply your program’s Standard Deviation function (e.g., Microsoft
Excel’s STDEV).
a. Calculate the Standard Deviation.
b. Calculate the sum of the OD values for all 384 wells, then divide by
384 to determine the Mean OD for the plate.
c. Calculate the %CV: (Standard Deviation / Mean OD) * 100.
The %CV should be <= 4.0.
If the %CV is greater than 4.0, one or more dispense tubes may need to be
cleaned. Run AutoClean and/or remove the manifold and use the stylus to
clean the dispense tube(s) giving lower-than-average absorbance readings.
When finished, re-prime and retry the test.
9. When finished, prime with deionized water to flush out the dye solution.
Evacuation Efficiency Test (192)
n This test is designed for the 192-Tube manifold. Find the Evacuation
Efficiency Test (96) on page 158.
n If you tared the balance at the start of the Dispense Precision Test, use the
plate from that test here; skip steps 1-3.
405™ Microplate Washer LS
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