66 | Chapter 3: Operation
Keypad
name
Option Description/Values range
Default
values
Pre-dispense
between
cycles
To wet or condition the manifold tubes
between cycles, which is only needed
after a long soak. Same parameters as
regular pre-dispense.
No
POST Post wash When all cycles are completed.
Final
Aspirate
A final aspiration is performed to
completely evacuate the wells. Same
parameters as regular aspirate step.
Yes
FORM Wash format Manner of processing large-format plates Plate
Sector Performs the entire wash step on one
sector of the plate before it moves to the
next sector.
Plate Performs each cycle to the entire plate
before it starts the next cycle.
Shake/Soak Step Parameters
Select the action to perform:
l Move carrier to home position first.
Regardless of selection, the plate carrier is moved home when the total time of the
shake and soak durations exceeds 1 minute. The vacuum pump is turned off in this
scenario. Moving the plate home prevents drops from contaminating the plate.
n Note: The Shake/Soak step is performed wherever the carrier is positioned. In
a multi-step protocol it is likely the step previous to a shake/soak will leave
the plate carrier in its home position. In this case and when it is the first step
in a protocol, the step will be performed at home.
l Shake settings:
l Duration: enter numeric values to shake the plate for up to 1 hour.
l Intensity: use the drop-down list to select a shake intensity. "Variable" cycles
through the other levels of intensity.
Intensity Hertz
Slow 7
Medium 13
Fast 19
405™ Microplate Washer LS
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