Washer Liquid Tests | 163
1. Fill a supply bottle with 2 liters of deionized or distilled water. Run the W-
DAY_RINSE protocol two or three times to prime the fluid lines and manifold.
2. Place a new 384-well microplate on the balance and zero the balance.
3. Pipette or dispense 80 μL of Solution #1 into each well of the microplate.
4. Place the plate on the carrier and run the QC-192_EVAC_TEST.This protocol
evacuates all of the wells, leaving a small amount of residual fluid.
5. When the program is completed, remove the plate and weigh it immediately
because evaporation will affect the results. This is the Total Residual Weight in
grams.
6. Visually inspect the plate and note if any wells appear to have considerably
more liquid in them than others.
7. Use the Evacuation Efficiency Test Worksheet to perform initial data
reduction:
1. Divide the Total Residual Weight by 384 to find the Mean Residual
Weight.
2. The Mean Residual Weight should be <= 0.002 g.
If the Mean Residual Weight is greater than 0.002 g, or if one or more wells
appear to have much more liquid than the others, the washer failed the test.
Troubleshoot as follows:
If the test fails once:
o
If the problem appears to be related to particular wells, clean those aspiration
tubes: run AutoClean and/or remove the manifold and thoroughly clean the tubes
with the stylus (See Remove and clean the washer manifold on page 131).
When finished, retry the test.
o
Failure of this test is commonly caused by improper aspiration tube placement
within the wells, usually because a microplate other than the recommended
Corning Costar
®
384 was used. If you must use a plate other than the Corning
Costar 384, modify the Aspirate Height, i.e., Z position, or horizontal X or Y
position parameters in a copy of the QC-192_EVAC_TEST protocol to correct this
error. After making this change, retry the test using a new microplate.
If the test fails a second time: Perform the Evacuation Diagnostic Test (192).
Evacuation Diagnostic Test (192)
n Conduct this test if the standard Evacuation Efficiency Test fails twice. This
test will confirm which aspirate tube(s) may be clogged, or if the plate’s
BioTek Instruments, Inc.
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