156 | Chapter 5: Qualification
Microplate Type Liquid Tests
Flat-bottom 384-well plates, Corning
Costar or equivalent
Evacuation Efficiency Test (192)
Dispense Precision Test (192)
Annual Buffer Switching Test
l 96-well 0.45 µm filter plate,
Millipore MSHVN4550 96 or
equivalent
l 384-well 1.2µm filter plate,
Millipore MZFCN0W10 or
equivalent
Vacuum Filtration Evacuation Efficiency
Test (Use the plate size most
commonly used in your lab)
l Precision balance with minimum capacity of 100 g and readability of 0.001 g
resolution
l Pipettes and graduated beakers
l Microplate absorbance reader capable of dual wavelength reading at 630/450
nm
l Liquid Test Worksheets at the end of this chapter for recordingdata and
results
l Deionized water
l Test solutions:
For this manifold: Sol. 1 Sol. 2 Sol. 3 Sol. 4
96-Tube 1000 mL 100 mL 1200 mL n/a
192-Tube 2000 mL 100 mL n/a 1440 mL
n These volumes are sufficient for performing the dispense tests and standard
and diagnostic Evacuation Efficiency tests. In most cases, enough fluid will be
left over to re-run a test, if necessary.
n If you will be performing the annual OQ for the external Buffer Switching
module, you will need several additional liters of deionized or distilled water.
n Verify™ Technology ("Q" models): if you will be qualifying level sensor
performance as part of the annual OQ, mix up another 200 mL of Solution #3
to run that test: Qualify the Verify™ Technology Sensor on page 167.
405™ Microplate Washer LS
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