102 | Chapter 3: Operation
LHC: Keypad:
and specify the vacuum duration, Time.
Optionally, do the same for the Final
Aspirate.
vacuum.
6. Select OPTS>MIDCYC and add
a Shake duration to follow each
dispense to suspend the beads
before applying the vacuum.
7. Select OPTS>POST to either
disable the Final Aspirate or
make sure it is defined for
vacuum filtration.
8. Experiment with other
parameters, like number of
cycles, to optimize the protocol
for your assay.
7. Fill the checkbox for Shake/Soak and
specify the shake duration.
Experiment with different settings to determine the optimal parameters to meet
your goals.
n Important: Be sure to change the Plate Carrier setting to match the installed
carrier.
Remember to specify the type of aspiration to perform in a Final Aspirate. Keypad
users: select OPTS>POST at the Wash Step Parameters menu.
405™ Microplate Washer LS
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