Predefined Sample Protocols | 45
W-NUNC_384 Standard wash protocols modified to best position the manifold tubes
for dispensing and aspirating to Nunc
®
flat-bottomed wells, round-
bottomed wells, and 384-well plates.
W-NUNC_FLAT
W-NUNC_
ROUND
Bead Assays - Biomagnetic Separation and Bottom Filtration
W-Luminex_
MAG_plate_96
Biomagnetic separation wash protocols designed for optimal bead
recovery in 96-well round- or flat-bottomed plates and 384-well plates
when using BioTek's Flat magnets. Begins by soaking the plate for 1
minute to let the beads settle at the bottom of the wells, then performs a
2 cycle wash with 30 second soaks between cycles.
Recommendation: Determine Magnet Height Offset on page 93 and apply
it for the plate you are using, especially when using 384-well plates.
W-Luminex_
MAG_384
W-Luminex_
VAC_96
Vacuum filtration wash protocols for Luminex
®
xMap polystyrene bead
assays using filter-bottom plates, 96- and 384-well. Protocol begins with
a 5 second bottom aspiration, followed by a 200 µL dispense for 96-well
plates and 75 µL dispense for 384-well, and a two cycle 200/75 µL wash.
W-Luminex_
VAC_384
BioTek Instruments, Inc.
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