Target Instrument Settings | 63
Keypad
name
Option Description/Values range
Default
values
Filtration
Time:
When applicable, specify duration of
vacuum filtration in seconds. 5-999
30
Travel Rate: The rate at which the washer manifold
travels down into the wells. The selection
range is 1 to 5 for non-cell-based assays,
from slowest to fastest. With these rates,
the tubes slow their descent as they
approach the defined aspirate height (Z
Position) to aid complete evacuation of
the well.
3
For delicate, cell-based assays, the range
is 1CW (cell wash) to 4CW and 6CW.
These rates minimize turbulence in the
wells. The tubes descend at a constant
rate to the specified height. Rate 6CW
creates the least disturbance and
performs fastest.
Delay: Amount of time the tubes stay at the
aspirate height before lifting out of the
wells. Define a delay between 0 - 5000
ms. Increasing the delay may improve
evacuation of the wells.
0
Positioning: X- and Y- horizontal axes, Z- height
(vertical) axis can be adjusted to improve
performance. Default Z-axis for 384-well
plates is 22 steps, 2 for 384-well PCR
plates.
Z = 29 for
96-well
plates;
X & Y = 0
Secondary
aspirate:
Also called Crosswise Aspiration. First the
wells are aspirated using the position
defined above. The aspirate tubes rise and
then descend to the secondary position to
aspirate again.
No
DISP Dispense
Flow Rate:
96-tube &
192-tube
manifolds
The rate at which the fluid is dispensed
from the tubes. For cell-based assays, use
rate 1 or 2 for gentle washing with the 96-
tube manifold only. For normal
dispensing, the range is 3-11, 3 is slowest
and 11 is fastest.
7
BioTek Instruments, Inc.
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