64 | Chapter 3: Operation
Keypad
name
Option Description/Values range
Default
values
Volume: µL/well dispensed range:
96-tube manifold: 50-3000
192-tube manifold: 25-3000
µL/well:
96= 300
384= 100
Buffer: Buffer bottle selection. A-D A
Positioning: X- and Y- horizontal axes, Z- height
(vertical) axis can be adjusted to improve
performance. Default Z-axis for 384-well
plates is 120 steps; 83 for 384-well PCR
plates.
Z = 121 for
96-well plate
Vacuum
Delay
Suspends the vacuum pump until a certain
volume is dispensed. This feature is
critical to cell wash operations. It delays
normal aspiration until the specified
volume has been dispensed to the wells.
The range is 10 to 350 µL/well.
10
OPTS Options
PRE Pre-wash options: Pre-dispense, Bottom
wash (if applicable)
MIDCYC Mid-cycle options: Shake, Soak, Pre-
dispense between cycles,
POST Post-wash option: Final Aspirate
FORM Format for washing 384-well plates: Plate
or Sector
PRE Pre-wash Actions performed before the wash cycles
begin
Pre-dispense Quick, small prime to condition the tips
before dispensing.
No
Flow Rate: 96-tube & 192-tube manifolds: The range
is 3-11, 3 is slowest and 11 is fastest.
9
Volume: µL/tube dispensed range:
96-tube manifold: 50-3000
192-tube manifold: 25-3000
µL/tube:
96 = 50
192 = 25
Bottom wash Bottom washing adds an initial wash cycle
to the specified number of cycles. Fluid is
No
405™ Microplate Washer LS
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