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Biotek
Washer
405 LS
Page 136
Biotek 405 LS - Page 136
240 pages
Manual
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Table of Contents
Main Page
Notices
4
All Rights Reserved
4
Trademarks
4
Restrictions and Liabilities
4
Table of Contents
5
Contact Information
10
Customer Service and Sales
10
Service/Tac
10
European Coordination Center/Authorized European Representative
10
Document Conventions
11
Document Revision History
11
Intended Use Statement
11
Quality Control
12
Warranty and Product Registration
12
Repackaging and Shipping
12
Warnings
12
Hazards and Precautions
13
Hazards
13
Precautions
14
CE Mark
16
Directive 2004/108/EC: Electromagnetic Compatibility
16
Directive 2006/95/EC Low Voltage (Safety)
17
Directive 2002/96/EC: Waste Electrical and Electronic Equipment
17
Directive 98/79/EC: in Vitro Diagnostics (if Labeled for this Use)
17
Electromagnetic Interference and Susceptibility
17
Usa Fcc Class a
17
Canadian Department of Communications Class a XVI
18
User Safety
18
Safety Symbols
19
Introduction
21
Introducing the 405™ Microplate Washer LS
22
Instrument Models
23
Biostack Compatibility
23
Package Contents
25
Waste and Dispense System Accessories
26
Optional Accessories
27
Physical Specifications
29
Performance Specifications
30
Biotek's Customer Resource Center
32
Installation
33
Unpack and Inspect the Instrument
34
Remove the Shipping Hardware
34
Setting up the 405 LS
38
Install the Microplate Carrier
38
Connect the Vacuum Pump, Tubes, and Bottles
38
Waste System
39
Install the Vacuum Line Filter
41
Install the Fluid Supply System
42
Final Check
44
Attach the Mist Shield
44
Install Software/Connect to Computer
45
Connect to Host Computer
45
Connect to Power
46
Define Instrument Settings
46
LHC Users Only
46
Standard Vacuum Pump Users
47
Define Startup Preferences (LHC Users Only)
47
Verify Performance
49
Verify the Washer
50
Run the Verify Test ("Q" Models Only)
50
Repacking the 405 Washer
52
Operation
57
Basic Operation
58
About the 405 LS Wash Step
58
Optimize Performance
59
405 Recommendations for Priming the Washer
59
Optimize Protocols to Improve Evacuation
60
Running Predefined Protocols
61
Creating Protocols: Washing, Aspirating and Dispensing Fluid
61
LHC Users Only: Customize the Predefined Protocols
61
Predefined Protocols Listing
63
Predefined Sample Protocols
64
Introducing the 405 LS Keypad
66
Quick Wash (Keypad Only)
67
Quick Prime (Keypad Only)
68
Create or Edit a Protocol (Keypad Only)
68
How to Name a Protocol (Keypad Only)
69
Define the Plate Type and Plate Map (or Partial Plate)
70
How to Shake the Plate
71
How to Enter Negative Numbers (Keypad Only)
72
How to Copy a Protocol
72
How to Delete a Protocol
72
Using LHC to Control the 405™ Washer
74
Introducing the LHC Workspace
74
Communications Port
75
405 Steps
75
LHC Protocols
76
LHC Protocols Explained
77
Target Instrument Settings
78
How to Define a Protocol (LHC Only)
79
How to Use the Adjust Utility- LHC
80
Upload-Download Protocols (LHC Only)
82
Wash Step Parameters Table
82
Shake/Soak Step Parameters
86
Pre-Dispense
87
Washer Flow Rates
88
How to Define a Final Aspiration Step
89
Add a Secondary Aspiration to a Wash Cycle
90
Bottom Wash
90
Verify Performance
92
Handling the Verify Test Plate
93
Verify™ Technology Flowchart Diagram
95
Verify Test Corrective Action
95
About Verify™ Technology
96
Sensitivity: Verify Sensor Vs. Absorbance Reader
98
Configure Verify™ Technology Utilities
99
Operating with the Biostack
101
Biostack Alignment Utility
102
Cell Wash
104
Cell Wash Strategies
105
Biomagnetic Separation - Magnetic Bead Assays
109
Perform Magnetic Bead Assays
110
Optimize Magnetic Bead Protocols
110
Flat Magnets
111
Ring Magnets
112
Magnet Height Offset
113
Determine Magnet Height Offset
113
Manifold Stop Screw Adjustment Kit
114
Vacuum Filtration for Filter Plate Assays
117
Install the Side Bracket
118
Set up the Vacuum Filtration Module
119
Enable Vacuum Filtration
121
Create a Vacuum Filtration Protocol
121
Install Vacuum Filtration Plate Carrier
123
Control the Vacuum Level
124
Washer Settings
125
Change the Washer Manifold Setting (Keypad)
127
Change the Plate Carrier Setting (Keypad)
127
Change the Plate Clearance Setting (Keypad)
128
Adjust Utility
128
Run the Adjust Utility (Using the Keypad)
129
Plate Types and Processing Patterns
130
Plate Types Table
131
About Wash Processing Patterns
131
Maintenance
135
Overview
137
Recommended Maintenance Schedule
139
Daily Maintenance
141
Overnight/Multi-Day Maintenance
141
Autoprime
142
Autoprime for Overnight-Weekend Maintenance
143
Turn on Autoprime for the Washer
143
Removing Protein Residuals and Fungi Growth
144
Autoclean the Washer
145
Quick Clean (Keypad Only)
146
Create an Autoclean Protocol
147
Periodic Maintenance
148
Clean the Bottles
148
Clean the Plate Carrier
149
Clean the Vacuum Filtration Carrier
149
Clean the Exterior Surfaces and Mist Shield
150
Clean the Buffer Bottle Filter
150
Remove and Clean the Washer Manifold
151
Maintaining the Vacuum Filtration System
153
Clean the Vacuum Filtration Carrier
153
Maintain the Verify™ Technology Components
153
Clean the Verify™ Level Sensor
153
Replacement Procedure for Verify Test Plate
154
Volume Coefficients - Slope and Offset
157
Decontamination
158
Tools and Supplies
158
Decontaminate Exterior Surfaces
159
Decontaminate Tubing and Manifold
160
Alternate Decontamination Procedure for Tubing and Manifold
161
Decontaminate the Vacuum Filtration System
162
Long Shutdown Procedure (Prepare for Storage or Shipment)
163
Storing the Instrument
164
Replace Components
164
Remove/Change Wash Manifold
165
Replace the Vacuum Pump Fuse
165
Vacuum Filtration Carrier: Replace the Gasket
166
Qualification
167
Qualification Overview
168
Qualification Schedule | 149
169
System Self-Test, Verify Information
169
Record Basecode
170
Sensor Test: Fluid, Waste, Plate Detection
170
Ultrasonic Cleaner Test
171
Liquid Testing the 405™ Microplate Washer LS
172
Which Tests to Perform
172
Important Recommendations for All Liquid Tests
173
Washer Liquid Tests
174
Dispense Precision Test
174
Evacuation Efficiency Test
174
Predefined Test Protocols
174
Washer Qualification Test Materials
175
Dispense Precision Test
177
Evacuation Efficiency Test
178
Evacuation Diagnostic Test
179
Buffer Switching Dispense Test
180
Evacuation Efficiency Test
182
Evacuation Diagnostic Test
183
Vacuum Filtration Evacuation Efficiency Test
185
Qualify the Verify™ Technology Sensor
187
Troubleshooting
199
Troubleshooting
200
General Operation Troubleshooting
202
Washer Problems
203
Communication Errors
208
Error Codes
211
Dispense Precision Test
212
System Error Codes
212
405 LS-LHC Software Error Codes
220
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