136 | Chapter 4: Maintenance
4. Put the Verify Test Plate back on the carrier and click OK to continue.
5. At the second pause, weigh the plate again and record the Dispense Weight
(W
D
) value.
6. Put the test plate back on the carrier and click OK to continue.
LHC users: A time and date stamped .txt file is created when the process is
completed.
Important: Touch screen users: Enter the weights onboard the washer when
prompted. The washer will perform the calculation described below and automatically
update the Slope and Offset. Skip the next two steps, but run the Verify routine as
advised in Step 9.
Format and evaluate test results:
7. LHC users only: In the Calibration Data folder, open the report. Copy and paste
all the data from the .txt file into the spreadsheet provided:
o
Select Ctrl+A and Ctrl+C
o
Select the From405 tab and paste (Ctrl+V) all the data into the worksheet
beginning at cell A1.
The slope and offset are calculated using these formulas:
Slope calculation:
Slope =
W W
D D
( − )
9600 × ( − )
D A
D A
Offset calculation:
Offset Slope D= − ( × )
W
D
0.096
D
o
D
D
is the Dispense Scan Mean value
o
D
A
is the Aspirate Scan Mean value reported in the date and time stamped .txt
file.
8. LHC users only: Review the current Slope and Offset values and change them to
match your calculated results, if they differ.
Touch screen LHC
To review the values that were updated by
this procedure:
1. Select Instrument>Other>Verify
Technology and select Advanced.
2. See the Volume Coefficients.
1. Select Tools>Instrument
Utilities>Verify
Manifold>Configure Utilities.
2. Click the Advanced settings link.
9. Run the Verify Manifold routine and confirm passing results.
Important: Write the 405 LS's serial number on the Verify Test Plate label.
405™ Microplate Washer LS
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