Biotek 405 LS - Page 188

168 | Chapter 5: Qualification
LHC
2. Click the link to Perform the Sensor Calibration.
3. At the first pausing message, weigh the plate and record the Aspirate Weight
(W
A
) value.
n When using Gen5, the spreadsheet does not open until the second read is
done. Jot down the weight for later entry in the spreadsheet.
4. Use the absorbance reader to read the plate at 630 nm and 450 nm.
l If you do not have Gen5, copy and paste the Delta transformation reading
results (630 nm - 450 nm) into the spreadsheet provided: in the Supporting
Data section of the worksheet, paste data into the Da (aspirate distance)
grid.
l If you have Gen5, the reading results will be reported later in the Power
Export.
5. Put the Verify Test Plate back on the 405 LS's carrier and select OK in the
pausing message to continue.
6. At the second pause, weigh the plate again and record the Dispense Weight
(W
D
) value.
7. Use the absorbance reader to read the plate at 630 nm and 450 nm. Gen5 users:
select OK.
l If you do not have Gen5, copy and paste the Delta transformation reading
results (630 nm - 450 nm) into the spreadsheet provided: in the Supporting
Data section, paste data into the Dd (dispense distance) grid.
l If you have Gen5, the Power Export containing the reading results opens. Fill
in the other data fields for weight and calibration data (as described below).
8. Put the test plate back on the 405 LS's carrier and select OK in the second
pausing message.
Format and evaluate test results:
9. Open the Calibration Data .txt file named in the final message (with the date and
time of execution) in the Calibration Data folder in the LHC folder structure.
1
10. Copy and paste all the data from the .txt file into the spreadsheet provided:
1
LHC users: The default location for this report is the Windows Common App Data folder (e.g.
C:\ProgramData) \BioTek\Liquid Handling Control version#\ManifoldTests\SN_##\Calibration Data. Touch
screen: transfer the memory stick to your PC.
405™ Microplate Washer LS