Mindray BS-130 - Page 250

10 Calculation Methods 10-7
21
1
VSV
SV
++
+
- double-reagent volume calibration factor
Double-wavelength (for both single-reagent and double-reagent)
The calculation method is similar to that for single-wavelength reaction, except that in
every measurement period the absorbance is the difference between primary wavelength
absorbance and secondary wavelength absorbance.
10.2.2.2 Calculating Response of Fixed-time Reaction
Single-wavelength (for both single-reagent and double-reagent)
bs
RRR － =
s
R and
b
R are calculated through
km
tt
tt
AA
R
km
−
−
= .
Where,
s
R - original response
b
R - reagent blank response.
b
R will be the response of the latest reagent blank. If no
reagent blank has been required,
b
R ＝0
l
t - start time for absorbance reading
m
t - end time for absorbance reading
Double-wavelength (for both single-reagent and double-reagent)
The calculation method is similar to that for single-wavelength reaction, except for every
measurement period, the absorbance is the difference between primary wavelength
absorbance and secondary wavelength absorbance.
10.2.2.3 Calculating Response of Kinetic Reaction
Single-wavelength (for both single-reagent and double-reagent)
bs
RRR － =
s
R and
b
R are calculated through the method of least squares.
Where,
s
R - original response
b
R - reagent blank response.
b
R will be the response of the latest reagent blank. If no
reagent blank has been required,
b
R ＝0
Formula with the method of least squares: