10 Calculation Methods 10-7
21
1
VSV
SV
++
+
- double-reagent volume calibration factor
Double-wavelength (for both single-reagent and double-reagent)
The calculation method is similar to that for single-wavelength reaction, except that in
every measurement period the absorbance is the difference between primary wavelength
absorbance and secondary wavelength absorbance.
10.2.2.2 Calculating Response of Fixed-time Reaction
Single-wavelength (for both single-reagent and double-reagent)
bs
RRR
-
=
s
R
and
b
R
are calculated through
km
tt
tt
AA
R
km
−
=
.
Where,
s
R
- original response
b
R
- reagent blank response.
b
R
will be the response of the latest reagent blank. If no
reagent blank has been required,
b
R
=0
l
t
- start time for absorbance reading
m
t
- end time for absorbance reading
Double-wavelength (for both single-reagent and double-reagent)
The calculation method is similar to that for single-wavelength reaction, except for every
measurement period, the absorbance is the difference between primary wavelength
absorbance and secondary wavelength absorbance.
10.2.2.3 Calculating Response of Kinetic Reaction
Single-wavelength (for both single-reagent and double-reagent)
bs
RRR
-
=
s
R
and
b
R
are calculated through the method of least squares.
Where,
s
R
- original response
b
R
- reagent blank response.
b
R
will be the response of the latest reagent blank. If no
reagent blank has been required,
b
R
=0
Formula with the method of least squares: