Mindray BS-130 User Manual

10 Calculation Methods
10-6
lg=
i
A
dibackgrouni
dibackgrouni
DD
DD
−
−
0
10.2.2 Calculating Response
For the system, the response (R) is defined as the absorbance change before and after
the reaction, or the absorbance change rate during the reaction process.
The formula for calculating the response (R) is closely related to the analytical method
(kinetic, fixed-time and endpoint), the number of reagents (single or double), and the
number of wavelengths (single or double). They are respectively detailed in the following
sections.
10.2.2.1 Calculating Response with Endpoint Method
Single-reagent and single-wavelength
SBs
RRR －=
s
R and
SB
R are calculated through
S
V
V
AAR
tt
+
×−=
−1
23
.
Where,
s
R - original response
SB
R response of sample blank. If no sample blank is required,
SB
R ＝0.
3
t
A - absorbance at
3
t
1
2
− t
A - absorbance at previous point of
2
t
S
V
V
+
- single-reagent volume calibration factor
Double-reagent and single-wavelength
bs
RRR － =
s
R and
b
R are calculated through
21
1
34
VSV
SV
AAR
ntt
++
+
×−=
−
.
Where,
s
R - original response
b
R - double-reagent blank response.
b
R is the response of the latest reagent blank.
4
t
A - absorbance at
4
t
nt
A
−
3
- absorbance at nt −
3
,
n
−
is the starting value of the reaction time

Mindray BS-130 Specifications

General

Brand	Mindray
Model	BS-130
Category	Measuring Instruments
Language	English