100687
Cell Test
BOD
Biochemical Oxygen Demand
Add 10 drops of
BOD-3K, reclose,
and mix.
Calculation:
BOD of measurement sample:
Result 1 – Result 2 (measurement sample) = A in mg/l
BOD of blank:
Result 1 – Result 2 (b
lank) = B in mg/l
BOD of original sample in mg/l = A
• dilution factor – B
Fill 2 oxygen reaction
bottles each with
pretreated sample
and 2 glass beads to
overflowing. Close
bubble-free with the
slanted ground-glass
stoppers.
Add 5 drops of BOD-1K
and then 10 drops of
BOD-2K, close bubble-
free, and mix for approx.
10 seconds.
Fill the solution into
a round cell.
Fill 2 oxygen reaction
bottles each with inocu-
lated nutrient-salt solu-
tion and 2 glass beads
to overflowing. Close
bubble-free with the
slanted ground-glass
stoppers.
Incubate one bottle
of pretreated sample
and one of inoculated
nutrient-salt solution
closed in a thermostatic
incubation cabinet at
20 + 1°C for 5 days.
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Use one bottle of
pretreated sample
and one of inoculated
nutrient-salt solution
for the measurement
of the initial oxygen
concentration.
After incubation, use
one bottle of pretreated
sample and one of
inoculated nutrient-
salt solution for the
measurement of the final
oxygen concentration.
Measurement of inital
oxygen concentration
= Result 1
(measurement sample)
= Result 1
(blank)
Measurement of final
oxygen concentration
= Result 2
(measurement sample)
= Result 2
(blank)
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) Spectroquant BOD
Standard (acc.
to EN 1899), Cat.No. 100718, can be used.
Preparation and incubation:
Determination:
Check the pH of the
sample, specified range
:
pH 6 – 8.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Reaction time:
1 minute
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Measuring 0.5 – 3000 mg/l BOD
range:
0.5 – 3000 mg/l O
2
Expression of results also possible in mmol/l.
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