Spectroquant Pharo 300 - Zero Adjustment

Spectroquant
®
Pharo 300 Operation
61
ba75703d07 04/2014
4.4 Zero adjustment
A valid zero adjustment is required for the calculation of measured values in
the modes, Concentration, Absorbance / % Transmission, Special / Multi
wavelengths and Kinetics. With a zero adjustment, the absorbance of a cell
filled with distilled water ("zero cell") is measured and stored.
Factory zero
adjustment for
concentration
measurements
For all measurements with Spectroquant
®
test sets (Concentration mode), a
factory zero adjustment is available in the delivery condition. We recommend
replacing it with a zero adjustment of your own.
Zero adjustment for
absorbance
measurements
In the Absorbance mode, the zero adjustment has to be carried out sepa-
rately for each cell type and each used wavelength. If a zero adjustment
exists already for the inserted cell type at the selected wavelength, the date
and time of the last zero adjustment are displayed in the top right area of the
display.
If no zero adjustment is available, the photometer will prompt you to carry out
a zero adjustment.
Note
The cells must be absolutely clean and free of scratches.
Always use a cell of the same type for zero adjustment and measurement of
the sample.
Notes on zero
adjustment
Zero adjustment with round cells:
 Only use clean, scratch-free round cells with distilled water. The minimum
filling level is 20 mm. A ready zero cell is included in the scope of delivery
of the photometer and PhotoCheck (see chapter 8 A
CCESSORIES AND
OPTIONS).
 A ready zero cell can, in principle, be used any number of times. We rec-
ommend, however, to regularly check the zero cell for visible contamina-
tion and scratches and refill or exchange it if necessary (at least every
24 months).
Zero adjustment with rectangular cells:
Absorbance
16.04.07 9:52
[ZERO 11.11.2010 11:11]
To start measurement, insert cell or press <START/
ENTER>
525 nm 10 mm
Setup Wavelength Transmission Reference