Spectroquant Pharo 300 - Measuring against the Reference Absorbance

Spectroquant
®
Pharo 300 Operation
103
ba75703d07 04/2014
4.6.3 Measuring against the Reference absorbance
Each time the photometer is switched on, the absorbance or transmission is
measured against the absorbance of the zero adjustment as a basis. You
can, however, also determine a Reference absorbance and use it as the
basis.
The Reference absorbance refers to the adjusted wavelength. The measured
value remains stored until
 the photometer is switched off
 the cell type is changed
 the wavelength is changed
 a new reference value is measured
 it is deleted manually ([Reference] / Delete).
 the Absorbance / % Transmission measuring mode is exited
Single and multiple
determination
The Reference absorbance can be determined with single or multiple deter-
mination. With multiple determination, the mean value is calculated as the
median from the individual measured values.
Measuring the
Reference
absorbance
The settings of the last measure-
ment are active.
1 Start the reference measurement
with [Reference].
If a value for the reference absor-
bance is already stored, it can be
deleted or overwritten by a new
reference measurement.
After the reference absorbance
value has been deleted, the pho-
tometer measures against the
absorbance of the zero adjust-
ment.
<HOME>
Absorbance / % Transmission
Absorbance
16.04.07 9:52
To start measurement, insert cell or press
<START/ENTER>
489 nm
10 mm
Setup Wavelength Transmission Reference