Pipette 10 ml of the
sample into a test tube.
Add 1 level blue micro-
spoon of Cl
2
-1.
Measuring 0.05 – 6.00 mg/l Cl
2
10-mm cell
range: 0.02 – 3.00 mg/l Cl
2
20-mm cell
0.010 – 1.000 mg/l Cl
2
50-mm cell
Expression of results also possible in mmol/l and also in free Cl
2
[Cl
2
(f)], combined Cl
2
[Cl
2
(b)], and
total Cl
2
[Cl
2
(t)].
Chlorine
Determination of free chlorine and total chlorine
100599
Test
Reaction time:
1 minute
Transfer the solution into
a corresponding cell.
Select method with
AutoSelector.
Place the cell into the
cell compartment.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) a freshly prepared
standard solution can be used (see section
“Standard
solutions”).
Determination of total chlorine
Same preparation as described above, add 2 drops of Cl
2
-2 and
mix after dissolving solid.
A differentiation between free and combined chlorine
[Cl
2
(f)
and Cl
2
(b)] can be performed on the photometer.
Prior to
measuring, select the differentiation measure-
ment and choose the corresponding citation form.
Then measure the free chlorine (result for “free Cl
2
” is
shown on the display), press enter and measure the
total chlorine. The individual measuring values for total
and combined chlorine are shown on the display.
Determination of free chlorine
Shake vigorously to
dissolve the solid
substance.
Check the pH of the
sample, specified range
:
pH 4 – 8.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Important:
Very high chlorine concentrations in the sample produce
yellow-colored solutions (measurement solution should be
red) and f
alse-low readings are yielded. In such cases the
sample must be diluted (plausibility check).
After each determination of total chlorine rinse the cell
with sulfuric acid 25 % and subsequently several times
with distilled water.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
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