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Spectroquant Pharo 300 - Page 365

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Pipette 200 ml of sample
into a separation funnel.
Add 5.0 ml of Ph-1 with
pipette and mix.
Add 1 level green micro-
spoon of Ph-2 and
shake to dissolve the sol-
id substance.
Reaction time:
30 minutes
(protected from light)
Measuring 0.002 – 0.100 mg/l C
6
H
5
OH 20-mm cell
range: Expression of results also possible in mmol/l.
Attention! The measurement is carried out in a 20-mm rectangular cell against a blank, prepared from distilled water
(Water for analysis EMSURE
®
, Cat.No. 116754, is recommended) and the reagents in an analogous manner.
Add 1 level green micro-
spoon of Ph-3 and
shake to dissolve the sol-
id substance.
Shake vigorously for
1 minute.
Transfer the clear lower
phase into a cell.
Select method with
AutoSelector measuring
range
0.002 – 0.100
mg/l
.
Place the cell into the
cell compartment.
Add 10 ml of chloroform
with pipette, close sepa-
ration funnel.
Leave to stand for
5 – 10 minutes to allow
the phases to separate.
Check the pH of the
sample, specified range
:
pH 2 – 11.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Phenol
100856
Test

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