Spectroquant Pharo 300 - Page 379

Pipette 5.0 ml of the
sample into a test tube.
Transfer the solution into
a corresponding cell.
Select method with
AutoSelector.
Place the cell into the
cell compartment
.
114842
Test
Phosphate
Determination of orthophosphate
Measuring 1.0 – 30.0 mg/l PO
4
-P 3.1 – 92.0 mg/l PO
4
2.3 – 68.7 mg/l P
2
O
5
10-mm cell
range: 0.5 – 15.0 mg/l PO
4
-P 1.5 – 46.0 mg/l PO
4
1.1 – 34.4 mg/l P
2
O
5
20-mm cell
Expression of results also possible in mmol/l.
Add 1.2 ml of PO
4
-1 with
piette and mix.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) ready-f
or-use
phosphate standard solution
Certipur
®
, Cat.No. 119898,
concentration 1000 mg/l PO
4
3–
, can be used after
diluting accordingly.
Check the pH of the
sample, specified range:
pH 0 – 10.
If required, add dilute
sulfuric acid drop by
drop to adjust the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Important:
For the determination of total phosphorus = sum of
orthophosphate,
polyphosphate and organophosphate
either Phosphate Cell Test, Cat. No. 114543, 114729, and
100673 or Phosphate Test, Cat. No. 114848 in conjunction
with Crack Set 10/10C, Cat. No. 114687 resp. 114688 can
be used.