Spectroquant Pharo 300 - Page 417

Pipette 10 ml of sample
into a glass vessel.
Add 1 level green micro-
spoon of Zn-1K and
shake to dissolve the sol-
id substance:
sample-reagent
mixture.
Pipette 0.50 ml of Zn-2K
into a reaction cell, close
with the screw cap, and
mix.
Reaction time:
15 minutes
100861
Cell Test
Measuring 0.025 – 1.000 mg/l Zn
range: Expression of results also possible in mmol/l.
Zinc
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Add 2.0 ml of the
sample-reagent
mixture with pipette,
close the cell with the
screw cap, and mix.
Add 5 drops of Zn-3K,
close the cell with the
screw cap, and mix.
Important:
For the determination of total zinc a pretreatment
with Crack Set 1
0C, Cat.No. 114688, or Crack Set 10,
Cat.No. 114687, and thermoreactor is necessary.
Result can be expressed as sum of zinc ( Zn).
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) ready-for-use
zinc standard solution Certipur
®
, Cat.No. 119806,
concentration 1000 mg/l Zn, can be used after diluting
accordingly.
Check the pH of the
sample, specified range
:
pH 1– 7.
If required, add dilute
sodium hydroxide
solution or hydrochloric
acid drop by drop to
adjust the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300