12 Software description
134 CyFlow
®
Cube 8 | Operating Manual | May 2018 | Revision 032
Controls group
The options in this group are available for single parameter overlay plots
only. They allow one plot within a gallery to be displayed as control on all
other plots within the gallery of the current overlay plot.
The Controls group contains the following options:
Set Control: The button is available when a single gallery plot is
selected. Click the button to display the plot as a control on all gallery
plots of the current overlay plot. The gallery plot set as a control displays
a dashed border.
If a control plot is already set, selecting another plot as a control will
replace the currently set control with the new selection on all plots within
the gallery.
Remove Control: The button is available when a plot is set as a control
for the gallery. This option removes the control plot from all plots in the
gallery.
Control Front: Select this option to display the control plot in front of the
plot data. Deselect the option to display the control plot in the
background of the plot data.
Calculations group
The buttons in this group are available when one or more single parameter
plots are selected in either the Overlay or Galleries area and the current plot
has a control already set.
When a control is set, two calculation options are available to compare test
and control plots: Overton or K-S comparison statistics. The selected
calculation will be applied to all plots within the selected overlay plot.
The Calculations group contains the following options:
None: The button is highlighted by default indicating that no calculation is
being displayed for the current plot.
Overton mode: The option shows the positive result after subtraction of
the control histogram from the test. The subtraction algorithm is a
cumulative subtraction routine based on that published by Roy Overton in
Cytometry 9:619-626 (1988).
K-S: The Kolmogorov–Smirnov test (K–S test) is a nonparametric test
method for comparing two samples, the control and test plots. The
maximum vertical deviation between the two curves is reported as the
statistic D.
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