12 Software description
86 CyFlow
®
Cube 8 | Operating Manual | May 2018 | Revision 032
Select the parameters for the gating plot: Select the parameters for
the primary bead / cell gating plot using the "X parameter" and "Y
parameter" boxes. They are populated with all the parameters shown in
the "Parameters" list.
Select background fluorescence mode: If no background fluorescence
control is present, select the "No background fluorescence" option. If a
separate background fluorescence control is being used, select the "Use
negative control file" option. If you wish to use negative events within a
positive sample to determine the background for each sample, select the
"Use negative regions" option.
Select the compensation plot type: Select whether histogram plots or
dual parameter density plots should be used for the definition of positive
and negative events for each parameter.
Allocate compensation parameters to listmode files: The list allows
you to allocate one of the parameters selected for compensation to each
LMD file. The listmode files (left-hand) side of the list shows the files from
the Playlist, in their current order, starting from the first file. The list
contains one LMD file for each selected compensation parameter. If the
"Use negative control file" option is selected, the list also contains a file to
be used as the negative control file. When the selection of parameters
changes, the list updates accordingly.
The allocated parameters (right-hand) side of the list shows the allocated
compensation parameters. Each row consists of a drop-down list that
contains all the fluorescence parameters from the "Parameters" list
selected for compensation. Selecting an item from the drop-down list
which is already selected for a different file clears the setting for that file.
The default allocation will be the negative control file first (if selected)
followed by the selected parameters in the order of the "Parameters" list.
If the listmode files list is updated (parameters selection or changing the
"Use negative control file" selection), the allocated parameters return to
their default allocations.
Next: Click the button to proceed to the next page of the Wizard. If the
"Use negative control file" option is selected, the next page is the
Background Fluorescence page, otherwise it is the first Compensation
page. If the selections made on the Configuration page are incorrect, you
will not be allowed to proceed to the next page, and an appropriate error
message will be displayed.
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