3.
Open the chip package, gently grasp the chip by its sides, then place it face-up
on the heated block, preheated to 40°C.
IMPORTANT! Allow the chip to sit on the heated block for at least 5 seconds
before loading it.
4.
Load the PCR reaction onto the chip:
a.
Briey vortex and centrifuge the prepared reaction (master mix, assay, and
template), then aspirate 14.5 µL of the solution into a P20 pipette. If the
reactions were placed on ice, allow them to warm prior to loading.
b.
While holding the loading blade by its edges, carefully dispense the
reaction into the sample-loading port. After loading, gently tap the top of
the loading blade to spread the reaction across the full length of the blade.
While lling the loading blade:
•
Hold the pipette so that the tip meets the sample-loading port at a
sharp angle and so it does not deect either blade (forward or rear).
•
Do not depress the pipette to the second stop. Doing so increases the
chance of introducing an air bubble to the loading blade.
•
Avoid creating air bubbles.
Appendix E Load chips manually
Load and seal the chips manually
E
QuantStudio
™
3D Digital PCR System User Guide
109
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