Thermo Scientific Applied Biosystems QuantStudio - Page 58

Observation Possible Cause Action
Condensation may have
been present on the chip
during imaging.
IMPORTANT! Condensation may
not be visible on the chip.
•
If condensation is not visible – If
possible, read the chip again
after it has warmed to room
temperature. Once equilibrated,
the data quality, ag, and
separation of amplied versus
non-amplied peaks should
improve noticeably.
•
If condensation is visible – Using
a low-lint wipe, remove any
condensation or Immersion Fluid
from the surface of the chip by
wiping in one direction. If
needed, use a low-lint wipe
sprayed with isopropanol to
remove any dried residue.
The loading blade was not
seated correctly within the
loader arm.
Before pressing the loading button,
conrm that the loading blade is rmly
seated and ush against the loader
arm.
Chapter 6 Troubleshooting
Troubleshooting chip images using the Chip View
6
58
QuantStudio
™
3D Digital PCR System User Guide