Operating the detector 3-41
EUFS set too low (flat-topped peak)
Auto-optimizing gain and EUFS
The auto-optimize gain diagnostic test allows the detector to run a single trial
chromatogram and suggests the ideal gain values to optimize the dynamic
range of the signal collection electronics. You must program a separation
method for the trial chromatogram. This can be done by entering a method via
the keypad or by retrieving a previously created method from a stored memory
location.
If you are using Empower or Millennium
32
software, you must enter the
method into the 474 Fluorescence Detector Instrument method editor. The
method is then downloaded to the 2475 detector when you make an injection.
After you program the method into the 2475 detector (or Empower or
Millennium
32
editor), press DIAG, and then press 3 Auto-Optimize Gain.
Selecting the auto-optimize gain diagnostic test
Minutes
Fluorescence
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