80 BD High Throughput Sampler User’s Guide
7 Click in the Acquisition Dashboard.
If necessary, choose View > Acquisition Dashboard.
The HTS homes and primes the probe, and then aspirates the unstained
control from the first setup control well.
NOTICE Setup and compensation wells are always acquired in standard
mode, even if high throughput mode is selected in the Plate window.
8 Adjust FSC, SSC, threshold, and PMTs using the unstained control.
For more information on optimizing cytometer settings, refer to the
appropriate cytometer user’s guide.
NOTICE Acquisition stopping time is determined by BD FACSDiva
software, according to sample volume/sample rate. BD recommends you set
the target number of events high enough so that you do not run out of
events before you reach the software-based acquisition stopping time. For
more information on stopping time, see Running Samples Automatically in
Sequence on page 46 and Running Wells Manually on page 47.
! Tip If you run out of sample before you finish optimizing settings, allow
the HTS to move to the next setup control well and continue optimization
from there.
9 Click Stop Well(s) once you have optimized settings.
The HTS goes through a purge cycle.
If the stopping time is met or the specified number of events are collected,
acquisition of the current well will stop, the HTS will be purged, and the
software will automatically proceed to the next well.
NOTICE No data file is saved for setup control wells.
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